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plasmid expressing both cas9 and single-guide rna (sgrna) targeting the tcf4 exon 2  (Addgene inc)


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    Addgene inc plasmid expressing both cas9 and single-guide rna (sgrna) targeting the tcf4 exon 2
    Plasmid Expressing Both Cas9 And Single Guide Rna (Sgrna) Targeting The Tcf4 Exon 2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/plasmid expressing both cas9 and single-guide rna (sgrna) targeting the tcf4 exon 2/product/Addgene inc
    Average 90 stars, based on 1 article reviews
    plasmid expressing both cas9 and single-guide rna (sgrna) targeting the tcf4 exon 2 - by Bioz Stars, 2026-03
    90/100 stars

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    TSP scp promotes gene activation activity of CRISPR-dCas system. ( A ) Activation of mNG expression by mCherry-dCas12f-VPR/sgRNA targeting mNG promoter in the HEK293T reporter cells after TSP scp /pDNA, TSP/pDNA, PEI/pDNA and Lipo/pDNA transfection. In the non-targeted (NT) sgRNA control, cells transfected with a non-targeted sgRNA. Fluorescence imaging and flow cytometry analysis were performed 48 h post transfection. Red, mCherry-dCas12f-VPR. Green, mNG. VPR (VP64-p65-Rta), a transcriptional activator. Scale bar, 250 μm. ( B ) Schematic analysis of endogenous gene activation by CRISPR-dCas12a delivered by TSP scp and other transfection reagents. ( C-D ) qPCR analysis of targeted genes including HBG, IL1RN and TTN, in TSP scp /pDNA, TSP/pDNA, PEI/pDNA and Lipo/pDNA transfected cells. RNA extraction was performed 48 h post transfection. n = 3 per group. Data represents as mean ± SD, ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001. P -values are from ANNOVA analysis and Sidak’s multiple comparisons test

    Journal: Journal of Nanobiotechnology

    Article Title: Short cell-penetration peptide conjugated bioreducible polymer enhances gene editing of CRISPR system

    doi: 10.1186/s12951-024-02554-w

    Figure Lengend Snippet: TSP scp promotes gene activation activity of CRISPR-dCas system. ( A ) Activation of mNG expression by mCherry-dCas12f-VPR/sgRNA targeting mNG promoter in the HEK293T reporter cells after TSP scp /pDNA, TSP/pDNA, PEI/pDNA and Lipo/pDNA transfection. In the non-targeted (NT) sgRNA control, cells transfected with a non-targeted sgRNA. Fluorescence imaging and flow cytometry analysis were performed 48 h post transfection. Red, mCherry-dCas12f-VPR. Green, mNG. VPR (VP64-p65-Rta), a transcriptional activator. Scale bar, 250 μm. ( B ) Schematic analysis of endogenous gene activation by CRISPR-dCas12a delivered by TSP scp and other transfection reagents. ( C-D ) qPCR analysis of targeted genes including HBG, IL1RN and TTN, in TSP scp /pDNA, TSP/pDNA, PEI/pDNA and Lipo/pDNA transfected cells. RNA extraction was performed 48 h post transfection. n = 3 per group. Data represents as mean ± SD, ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001. P -values are from ANNOVA analysis and Sidak’s multiple comparisons test

    Article Snippet: Plasmids expressing single guide RNA (sgRNA) were constructed by ligating the corresponding annealed oligos to the basic plasmid under the human U6 promoter (GENEWIZ, China).

    Techniques: Activation Assay, Activity Assay, CRISPR, Expressing, Transfection, Fluorescence, Imaging, Flow Cytometry, RNA Extraction